처리결과

Ected exclusively in roots of the tolerant genotype (Prata Ligeiro) SSH library (Continued)Unknow function Hypothetical protein Hypothetical protein Conserved hypothetical protein Hypothetical protein Hypothetical protein Hypothetical protein Hypothetical protein Hypothetical protein Hypothetical protein Hypothetical protein Hypothetical protein Hypothetical protein Hypothetical proteinOryza sativa Oryza sativa Oryza sativa Oryza sativa Oryza sativa Oryza sativa Oryza sativa Oryza sativa Oryza sativa Oryza sativa Oryza sativa Oryza sativa Oryza sativa Oryza sativaNM_001067277 AP008208 gb|EAY93896.1| NM_001065538 gb|EAY84091.1| CT836006 NC_008394.1 NC_008394.1 AP008208 NM_001057688 NM_001066910 NM_001053573 CT829595 CTIn this study we have also identified genes which have not yet been directly related to drought tolerance, such as B12Dg1 protein, Nuclear protein SET domain Nelfinavir (Mesylate) containing protein and Putative pollen specific protein C13 precursor, as well as genes with unknown function. Further studies need to be performed in order to assign biological function, since these genes may play important roles in plant adaptation during drought stress conditions.2.2. Drought-responsive genes identified in IRAT20 Regarding the response of the susceptible genotype to PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/25751659 drought stress, 71 transcripts were exclusively expressed in this genotype. As in Prata Ligeiro, a high number of genes (14) with no known homologs (no hits) were identified (Table 2). Moreover, a total of 23 genes encoding hypothetical or unknown proteins were also observed. Further expression studies of these genes may reveal important genes associated to drought stress response, which have not been explored so far. This information may contribute to a better understanding of the mechanisms related to drought susceptibility in upland rice varieties.3. Proteome analysis In order to complement the genomic studies, protein maps of roots from water-stressed plants of the susceptible (Figure 1A) and tolerant (Figure 1B) genotypes were compared. Triplicates of the gels from each genotype were compared and revealed a total of 463 proteins in the Prata Ligeiro profile and 522 in IRAT20. The two obtained synthetic gels were overlapped and this procedure allowed the identification of 307 overlapped spots, 156 proteins exclusive to the tolerant genotype and 215 proteins exclusive to the susceptible genotype. These results show a higher diversity in the protein pattern of the susceptible genotype.As in Prata Ligeiro, three transcripts showed similarity to non-plant proteins and were not considered in the analysis since they probably represent contaminating sequences (Table 2). The other transcripts showed similarity to genes associated to different functions including the transport of small molecules or inorganic ions, such as HCO3-transporter and Vacuolar H+ pyrophosphatase. The expression of these genes was previously reported by Wang et al. [23] in a lowland variety. These results suggest that upland genotypes susceptible to drought may present similar responses to those of lowland varieties, which are naturally more susceptible to water deficit. Interestingly, the well-known transcription factor WRKY was uniquely identified in IRAT20. WRKY mediates plant stress responses [52-54] and the increased expression of this protein has been frequently associated to drought stress response in rice [23,55].A total of 50 intense proteins observed in the tolerant genotype profile after Coomassie blue stain.